II. Indications: Evaluation of Bacterial Infection
- Bacteremia
- Sepsis
III. Technique
- Preparation of skin
- Chlorhexidine (preferred agent for antiseptic)
- Superior to Povidone-Iodine
- Superior to Alcohol
- Povidone-Iodine
- Wait to draw blood for 2-3 minutes after applying
- High contamination rate compared with Chlorhexidine
- References
- Chlorhexidine (preferred agent for antiseptic)
- Standard collection protocol
- Collect up to 3-4 sets of Blood Culture bottles
- Two Blood Culture bottles in each set
- Obtain 5 ml blood per bottle
- Draw from up to 3 different venipuncture sites
- Space blood collections by 1 hour intervals
- Collect up to 3-4 sets of Blood Culture bottles
- New collection protocol: Six bottle method
- Collect 6 bottles from one venipuncture (total 40 ml)
- Based on mathematical model
- Predicts highest sensitivity and Specificity
- References
IV. Efficacy
- Sensitivity
- Increases with number of Blood Cultures drawn
- With 3 Blood Cultures, Sensitivity approaches 90%
- Study of hospitalized patients with persistent fever
- Post-Antibiotic culture unlikely to yield pathogen
- Obtain pre-antibiotic Blood Culture and await results
- Grace (2001) Clin Infect Dis 32:1651-5 [PubMed]
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Concepts | Laboratory Procedure (T059) |
ICD9 | 90.52 |
SnomedCT | 168333009, 145540002, 30088009 |
English | Blood culture, blood culture (lab test), blood culture, Culture blood, Culture-blood, Haemoculture, Hemoculture, of blood culture, blood cultures, blood culture procedure, blood culture of, culture blood, Microscopic examination of blood, culture, Culture of blood, Blood Culture, BC - Blood culture, Blood culture (procedure), Blood culture, NOS |
Italian | Emocoltura |
Dutch | kweek bloed, hemocultuur, bloedkweek |
Spanish | Cultivo de sangre, Hemocultivos, cultivo de sangre, SAI, cultivo de sangre, hemocultivo (procedimiento), hemocultivo, Hemocultivo |
Japanese | 血液培養, ケツエキバイヨウ |
Czech | Hemokultura |
German | Haemokultur, Blutkultur |
Hungarian | Haemocultura, Haemotenyészet, Hemotenyészet, Hemokultúra |
Portuguese | Hemocultura |
French | Hémoculture |