II. Background
- Lipid rich cell walls (Mycobacterium, Nocardia) retain red stain on Acid Fast Staining
III. Technique
IV. Interpretation
- Acid Fast Positive
- Lipid-rich cell walls retain red stain
- Acid Fast Bacilli
- Mycobacterium species
- Nocardia species (partially acid-fast, also considered Gram Positive)
- Non-Bacteria may also be acid-fast (e.g. Bacterial endospores, Cryptosporidium parvum)
- Acid Fast Negative
- Non-Acid Fast Bacteria instead stain blue
V. Resources
- Acid Fast Bacteria (StatPearls)
- Acid Fast Stain Protocol (American Society of Microbiology)
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Related Studies
| Concepts | Bacterium (T007) |
| SnomedCT | 39701000009103 |
| English | acid-fast bacteria, acid bacteria fasting, acid fast bacteria, acid bacteria fast, Acid-fast bacteria, Acid-fast bacterium (organism), Acid-fast bacterium |
Ontology: Acid fast stain positive (C0855688)
| Concepts | Laboratory or Test Result (T034) |
| English | Acid fast stain positive |
| Spanish | Tinción acidorresistente positiva |
| Portuguese | Coloração acidorresistente positiva |
| Italian | Colorazione acido-resistente positiva |
| French | Coloration acidorésistante (Ziehl-Neelsen) positive |
| German | saeureechte Faerbung positiv |
| Dutch | zuurvaste kleuring positief |
| Czech | Barvení na acidorezistentní tyčinky pozitivní |
| Japanese | 抗酸染色陽性, ｺｳｻﾝｾﾝｼｮｸﾖｳｾｲ |
| Hungarian | Saválló festés pozitív |
Ontology: Acid fast stain (C1318720)
| Concepts | Indicator, Reagent, or Diagnostic Aid (T130) |
| SnomedCT | 406976001 |
| English | acid fast stain, acid fast, acid fast stains, fast acid stain, acid fast staining, Acid fast stain (substance), Acid fast, Acid fast stain |
| Spanish | colorante acidorresistente (sustancia), colorante acidorresistente |
Ontology: Ziehl-Neelsen stain method (C1318721)
| Definition (HL7V3.0) | <p>Stain, Acid fast, Ziehl-Neelsen</p> |
| Definition (NCI_CDISC) | A microscopy acid fast staining method that utilizes Ziehl-Neelsen carbol fuchsin, acid alcohol and methylene blue, to determine the presence of acid fast microorganisms. This acid fast staining technique requires heating during the primary staining step involving the carbol fuchsin. |
| Definition (NCI) | A microscopy acid fast staining method that utilizes Ziehl-Neelsen carbol fuchsin, acid alcohol and methylene blue, to determine the presence of acid fast microorganisms. This acid fast staining technique requires heating during the primary staining step involving the carbol fuchsin. |
| Concepts | Laboratory Procedure (T059) |
| SnomedCT | 48493000, 406979008 |
| HL7 | 0146 |
| LNC | LP6097-2 |
| Spanish | tinción acidorresistente, método de Ziehl - Neelsen, método de tinción acidorresistente con técnica de Ziehl - Neelsen (procedimiento), método de tinción acidorresistente con técnica de Ziehl - Neelsen, tinción acidorresistente, método de Ziehl - Neelsen (procedimiento), método de tinción de Ziehl-Neelsen (procedimiento), método de tinción de Ziehl-Neelsen |
| English | Acid fast stain, Ziehl-Neelsen method, Ziehl Neelsen Acid Fast Staining Method, Acid fast stain.Ziehl-Neelsen, Acid fast Ziehl-Neelsen stain method (procedure), Acid fast Ziehl-Neelsen stain method, Acid fast Ziehl-Neelsen stain, ZIEHL NEELSEN ACID FAST STAIN, Ziehl-Neelsen stain, Ziehl-Neelsen stain method (procedure), Ziehl-Neelsen stain method, Acid fast stain, Ziehl-Neelsen method (procedure), Acid fast stain, Ziehl-Neelsen |
