Peripheral Smear

Aka: Peripheral Smear, Peripheral Blood Film, Peripheral Blood Smear, Blood Film

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II. Technique: Staining

  1. Staining
    1. Avoid water contamination
    2. Romanowsky variants based on methylene blue and eosin
      1. Wright Stain (Sodium Bicarbonate)
      2. May-Grunwald-Giemsa Stain (acid bichromate)
  2. Slide evaluation
    1. Evaluate for adequate sample under 10x power
    2. Cell exam under oil immersion lens at 50x or higher

III. Labs

  1. See Erythrocyte Morphology
  2. See Leukocyte Morphology
  3. See Platelet Morphology

IV. References

  1. Lee (1999) Wintrobe's Hematology, Lippincott, p. 23-25

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