II. Collection
- Sterile Methods
- Catheterized Urine
- Suprapubic Tap
- Clean Methods
III. Specimen handling
- Volume: 5 ml (0.2 ml minimum)
- First morning void is ideal specimen
- Deliver to lab or refrigerate within 30 minutes
IV. Interpretation: Clean Voided Specimen
- Normal: <10,000 organisms per ml
-
Urinary Tract Infection
- Boys
- Organisms >10,000 per ml suggests UTI likely
- Girls
- Organisms >100,000 per ml suggests UTI
- Three specimens: 95% probability of UTI
- Two specimens: 90% probability of UTI
- One specimen: 80% probability of UTI
- Organisms >10,000 per ml needs repeat Urine Culture
- Organisms >100,000 per ml suggests UTI
- Boys
V. Interpretation: Transurethral Catheterization
- Normal: <1,000 organisms per ml
-
Urinary Tract Infection
- Organisms >10,000 per ml suggests UTI
- Organisms >1,000 per ml needs repeat Urine Culture
VI. Interpretation: Suprapubic aspiration in infants
- Gram Negative Bacilli: any number suggests UTI (99%)
- Gram Positive Cocci: >1-2,000 suggests UTI (99%)