II. Precautions
- Infected Chronic Wounds may not present with typical infectious findings- Erythema, edema, pain and fever may be absent despite infection
 
III. Signs: Chronic Wound Colonization and Infection
- See Chronic Wound
- 
                          Chronic Wound Biofilm or Critical Colonization (Mnemonic: NERDS; Test Sensitivity 73%, Test Specificity 80%)- Non-Healing Wound
- Exudate
- Red with easy bleeding (friable)
- Debris
- Smell
 
- Infected Chronic Wound (Mnemonic: STONEES; Test Sensitivity 90%, Test Specificity 69%)- Size Increasing
- Temperature increased (fever)
- Os (Probe To Bone Test positive)
- New areas of tissue breakdown
- Exudate
- Erythema or Edema
- Smell
 
IV. Labs
- Biopsy with Bacterial Counts (preferred biopsy method)- Positive if >100,000 colony forming units in at least one sample
 
- Levine Technique (second best method)- Debride superficial necrotic tissue
- Apply pressure with cotton swab rolled over a 1x1 cm area for 5 seconds
- Culture the swab
 
- Z-Technique- Swab is rotated between fingers as it passed over the surface of wound in zig-zag pattern
- Avoid touching the wound edges with the cotton swab
 
