II. Indications
- Confirmation of an indeterminate or positive less-specific lab result (e.g. ELISA)
III. Mechanism
- Detects Proteins and peptides separated by gel electrophoresis and identified with labeled antibodies
IV. Technique
- Sampled cells are lysed and Protein extracted (e.g. from patient blood sample)
- Extracted Proteins are applied to a gel surface
- Electrical current applied to gel matrix separates molecules based on charge and mass
- Separated molecules are transferred from gel onto paper or nylon membrane (e.g. nitrocellulose)
- Radiolabeled or Enzyme-linked Antibody probes (similar to ELISA) detect specific substances