II. Definitions
- Neutralization Test
- Antibody or Antigen activity tested through sequential dilution until activity ceases
- Activity test examples include Infectivity in tissue culture, Hemolysis or Agglutination
III. Types
- Plaque Reduction Neutralization Test (PRNT)
- Patient serum sample at sequential dilutions added to known virus sample and then incubated
- Solution of serum and virus are poured over a single layer of host cells and covered with agar
- Concentration of infected cell regions (Plaques) are then measured over several days
- Complement Fixation Test
- Patient serum sample at sequential dilutions added to known virus sample
- Test solution contains complement and RBCs (e.g. sheep RBCs) coated with hemolysin
- Serum-virus solution is added to the complement-RBC test solution
- Antistreptolysin O Titer (ASO Titer)
- ASO Titer is now performed as an Agglutination Test, but was previously a Neutralization Test
- Streptococcus produces streptolysin O which causes Hemolysis
- Test starts by combining streptolysin O with sequential dilutions of patient serum
- Human RBCs are added to streptolysin O and serum mix
- Antistreptolysin Antibody will neutralize the streptolysin and prevent Hemolysis
- Maximal serum dilution that still prevents Hemolysis will result in an ASO Titer
IV. Resources
- Plaque Reduction Neutralization Test (Wikipedia)
V. References
- Goldberg (2014) Clinical Physiology, Medmaster, Miami, p. 83-5
Images: Related links to external sites (from Bing)
Related Studies
Definition (MSH) | Serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1). Binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2). Failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1. If red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1. |
Definition (CSP) | serologic tests based on inactivation of complement by the antigen-antibody complex (stage 1); binding of free complement can be visualized by addition of a second antigen-antibody system such as red cells and appropriate red cell antibody (hemolysin) requiring complement for its completion (stage 2); failure of the red cells to lyse indicates that a specific antigen-antibody reaction has taken place in stage 1; if red cells lyse, free complement is present indicating no antigen-antibody reaction occurred in stage 1. |
Concepts | Laboratory Procedure (T059) |
MSH | D003168 |
SnomedCT | 69207009 |
English | Fixation Test, Complement, Fixation Tests, Complement, Test, Complement Fixation, Tests, Complement Fixation, Complement Fixation Test, complement fixation test, complement fixation tests, Complement fixation test, Complement fixation test (procedure), Complement fixation test, NOS, Complement Fixation Tests |
Swedish | Komplementfixeringstest |
Czech | komplement - fixační testy |
Finnish | Komplementinsitoutumiskokeet |
French | Tests de fixation du complément, Réactions de fixation du complément, RFC (Réaction de Fixation du Complément) |
Russian | KOMPLEMENTA SVIAZYVANIIA TESTY, KONGLIUTINATSII REAKTSIIA, KONGLIUTINIRUIUSHCHII KOMPLEMENT, ABSORBTSIONNYI TEST, КОМПЛЕМЕНТА СВЯЗЫВАНИЯ ТЕСТЫ, КОНГЛЮТИНАЦИИ РЕАКЦИЯ, КОНГЛЮТИНИРУЮЩИЙ КОМПЛЕМЕНТ, АБСОРБЦИОННЫЙ ТЕСТ |
Japanese | 補体吸収テスト-膠着, 膠着補体吸収テスト, 補体結合反応, 膠着反応, 補体結合試験 |
Croatian | KOMPLEMENT, VEZANJE, TESTOVI |
Polish | Test wiązania dopełniacza konglutynacyjny, Testy wiązania dopełniacza, Odczyn koaglutynacyjny, CFT, Próby wiązania dopełniacza, Reakcja konglutynacji |
Norwegian | Komplementfiksasjonsprøver, Komplementbindingsprøver |
Spanish | Tests de Fijación de Complemento, prueba de fijación del complemento (procedimiento), prueba de fijación del complemento, Pruebas de Fijación del Complemento |
German | Komplementbindungsreaktionen |
Italian | Prove di fissazione del complemento |
Dutch | Complementbindingstest |
Portuguese | Testes de Fixação de Complemento |
Ontology: Neutralization Tests (C0027944)
Definition (MSH) | The measurement of infection-blocking titer of ANTISERA by testing a series of dilutions for a given virus-antiserum interaction end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology (CPE) or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). |
Definition (CSP) | titration of an antiserum by testing a series of dilutions of virus or immune serum to a given end-point, which is generally the dilution at which tissue cultures inoculated with the serum-virus mixtures demonstrate cytopathology or the dilution at which 50% of test animals injected with serum-virus mixtures show infectivity (ID50) or die (LD50). |
Concepts | Laboratory Procedure (T059) |
MSH | D009500 |
LNC | LP6416-4 |
English | Neutralization Test, Test, Neutralization, Tests, Neutralization, antibody neutralization test, neutralization test, Neut, Neutralization Tests |
Swedish | Neutralisationstester |
Czech | neutralizační testy |
Finnish | Neutralisaatiokokeet |
French | Tests de neutralisation, Réactions de neutralisation |
Russian | NEITRALIZATSII TESTY, НЕЙТРАЛИЗАЦИИ ТЕСТЫ |
Croatian | NEUTRALIZACIJSKI TESTOVI |
Polish | Próby neutralizacji, Testy neutralizacji |
Norwegian | Nøytraliseringstester |
Spanish | Tests de Neutralización, Pruebas de Neutralización |
German | Neutralisationstests |
Italian | Prove di neutralizzazione |
Dutch | Neutralisatie-onderzoek, Onderzoek, neutralisatie- |
Portuguese | Testes de Neutralização |