Hematology and Oncology Book

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Coombs

Aka: Coombs, Coombs Reagent
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  1. Coombs Reagent
    1. Anti-Human Globulin Antibody
    2. Binds to human IgG Antibody and C3 Complement
    3. Produced in rabbits by Immunization
  2. Direct Coombs
    1. Question
      1. Is IgG or complement bound to Red Blood Cell?
    2. Technique
      1. Start with patient's Red Blood Cells
      2. Add Anti-human globulin antibodies (Coombs Reagent)
    3. Direct Coombs Positive
      1. Agglutination indicates Antibody coated RBCs
    4. Example of use
      1. Are maternal antibodies bound to fetal RBCs?
  3. Indirect Coombs
    1. Question
      1. Is Antibody against Red Blood Cell present in serum?
    2. Technique
      1. Start with patient's serum
      2. Add Anti-human globulin antibodies (Coombs Reagent)
      3. Add Indicator Red Blood Cells with known antigens
    3. Indirect Coombs Positive
      1. Agglutination indicates Antibody to RBC in serum
      2. Antibody titer can be obtained by serial dilution
    4. Example of use
      1. Are antibodies to fetal RBC present in maternal serum

Coombs' Test (C0009961)

Definition (NCI) A laboratory test to identify antibodies that can bind to the surface of red blood cells or platelets and destroy them. This test is used to diagnose certain blood disorders in which patients make antibodies to their own red blood cells or platelets. It is also used to determine blood type.
Definition (MSH) Hemagglutination test in which Coombs' reagent (antiglobulin, or anti-human globulin rabbit immune serum) is added to detect incomplete (non-agglutinating, univalent, blocking) antibodies coating erythrocytes. The direct test is applied to red cells which have been coated with antibody in vivo (e.g., in hemolytic disease of newborn, autoimmune hemolytic anemia, and transfusion reactions). The indirect test is applied to serum to detect the presence of antibody (e.g., in detection of incompatibility in cross-matching tests, detection and identification of irregular antibodies, and in detection of antibodies not identifiable by other means).
Definition (NCI) Hemagglutination test in which Coombs' reagent (antiglobulin, or anti-human globulin rabbit immune serum) is added to detect incomplete (non-agglutinating, univalent, blocking) antibodies coating erythrocytes. The direct test is applied to red cells which have been coated with antibody in vivo (e.g., in hemolytic disease of newborn, autoimmune hemolytic anemia, and transfusion reactions). The indirect test is applied to serum to detect the presence of antibody (e.g., in detection of incompatibility in cross-matching tests, detection and identification of irregular antibodies, and in detection of antibodies not identifiable by other means). (MeSH)
Concepts Laboratory Procedure (T059)
MSH D003298
SnomedCT 143184007, 165778006
English Anti-Human Globulin Consumption Test, Antiglobulin Consumption Test, Antiglobulin Consumption Tests, Antiglobulin Test, Antiglobulin Tests, Antihuman Globulin Consumption Test, Consumption Test, Antiglobulin, Consumption Tests, Antiglobulin, Coomb Test, Coomb's Test, Coombs' Test, Test, Antiglobulin, Test, Antiglobulin Consumption, Test, Coombs', Tests, Antiglobulin, Tests, Antiglobulin Consumption, Coombs' test, Coombs test NOS, Coombs, ANTIHUMAN GLOBULIN CONSUMPTION TEST, Coombs test (lab test), Coombs test NOS (procedure), Antiglobulin test, coombs test, test coombs, Test;Coombs, coomb test, coombs testing, coombs' test, coomb's test, Coomb's test, antiglobulin test, Anti Human Globulin Consumption Test, Coombs test, Coombs Test
Italian Test di Coombs, Test di Coombs NAS, Test di consumo dell'antiglobulina, Test dell'antiglobulina, Prova di Coombs
Dutch Coombs-test NAO, Antiglobulineconsumptietest, Antiglobulinetest, Coombs-test, Globulineconsumptietest, Antihumane, Test, Coombs-
French Test de Coombs SAI, Test de Coombs, Test à l'antiglobuline, Test de consommation de globuline anti-humaine, Test de consommation d'antiglobuline
German Coombs Test NNB, Coombs Test, Anti-Humanglobulin-Verbrauchstest, Antiglobulin-Verbrauchstest, Antiglobulintest, Coombs-Test
Portuguese Teste de Coombs NE, Teste de Coombs, Teste de Antiglobulina, Teste de Consumo de Antiglobulina, Teste de Consumo de Globulina Anti-Humana
Spanish Prueba de Coombs NEOM, Coombs test NOS, prueba de Coombs, SAI (procedimiento), prueba de Coombs, SAI, Test de Antiglobulina, Test de Consumo de Antiglobulina, Test de Consumo de Globulina Antihumana, Test de Coombs, Prueba de Coombs
Japanese クームス試験ＮＯＳ, ｸｰﾑｽｼｹﾝ, ｸｰﾑｽｼｹﾝNOS, Coombsテスト, クームステスト, クームス消費試験, クームス試験, 抗グロブリンテスト, 抗グロブリン消費テスト, 抗グロブリン消費試験, 抗グロブリン試験, 抗ヒトグロブリン消費テスト, Coombs試験
Swedish Coombs' test
Czech Coombsův test, Coombsův test NOS
Finnish Coombsin koe
Russian ANTIGLOBULINOVYI TEST, KUMBSA TEST, АНТИГЛОБУЛИНОВЫЙ ТЕСТ, КУМБСА ТЕСТ
Polish Test Coombsa, Próba antyglobulinowa, Test antyglobulinowy
Hungarian Coombs vizsgálat, Coombs vizsgálat k.m.n.
Sources
Derived from the NIH UMLS (Unified Medical Language System)


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