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Urine Microscopic Exam

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Urine Microscopic Exam, Microscopic Urinalysis

  • See Also
  • Preparation
  • Sample
  1. Obtain fresh urine sample
  2. Centrifuge 10-15 ml at 1500 to 3000 rpm for 5 minutes
  3. Decant supernatant and resuspend remainder of urine
  4. Place 1 drop of urine on slide and apply cover slip
  • Exam
  1. Urine Cells
    1. Urine White Blood Cells
      1. Normal <2/hpf in men and <5/hpf in women
    2. Urine Red Blood Cells
      1. Normal <3/hpf
      2. Dysmorphic RBCs suggest glomerular disease
    3. Epithelial cells
      1. Transitional epithelial cells are normally present
      2. Squamous epithelial cells suggest contamination
      3. Renal tubule epithelial cells suggest renal disease
  2. Bacteria
    1. Five Bacteria per hpf represents 100,000 CFU/ml
    2. Diagnostic for Urinary Tract Infection
      1. Men: Any Bacteria
      2. Women: 5 or more Bacteria per hpf
  3. Urine Crystals
    1. See Urine Crystals
  4. Urine Casts
    1. See Urinary Cast