Lab

Chlamydia Antigen

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Chlamydia Antigen

  • Indication
  1. Chlamydia screening
  • Detection
  1. Methods
    1. Direct Fluorescent Antibody (DFA)
    2. Enzyme Immunoassay (EIA)
  2. Mechanism
    1. Detects both live and dead organisms
    2. Positive test may be seen in recent treatment
  • Technique
  1. Eye Sample
    1. Topical Eye Anesthetic applied
    2. Swab lower and upper lids (inner surface)
  2. Urethral Samples (Males)
    1. Patient should not urinate within 1 hour before test
    2. Insert small Dacron swab 2-4 cm into Urethra
  3. Cervical Samples
    1. Wipe Cervix to remove excess mucus
    2. Insert Dacron swab into endocervix
    3. Rotate swab for 5 to 10 seconds
    4. Withdraw swabs without touching any vaginal surface
  4. Rectal Samples (symptomatic patients)
    1. Insert Dacron swab 3 cm into anal canal
    2. Withdraw swab
    3. Fecal contaminated samples should be redone
  • Efficacy (DFA, EIA)
  1. Sensitivity: 88-91%
  2. Specificity: 97-99%