Lab
Platelet Function Closure Time
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Platelet Function Closure Time
, Platelet Function Analyzer, PFCT, PFA-100
Indication
Bleeding Disorder
Replaced by von specific tests (Willebrand Factor Activity,
Von Willebrand Factor Antigen
, Factor VIII Level)
Historical indications
Replaced
Bleeding Time
to assess platelet function
Personal or
Family History
of easy
Bruising
or bleeding
Epistaxis
Menorrhagia
Post-operative bleeding
Protocol
Sample
Citrate anticoagulated whole blood
Mechanism
Rapid quantitation of platelet function for hemostasis
Blood passed through high shear flow system
System measures time to occlude membrane
Blood is tested with two membranes
Collagen/
Epinephrine
(CEPI) membrane
Collagen/
Adenosine
Diphosphate (CADP) membrane specific for
Aspirin
Interpretation
Normal
CEPI closure <175
Causes
Abnormal (prolonged closure time)
Von Willebrand's Disease
Glanzmann's
Thrombasthenia
Thrombasthenia
Thrombocytopenia
with
Platelet Count
<100,000/mm3
Anemia
with
Hematocrit
<35%
Aspirin
(will prolong only the CEPI membrane time)
References
Harrison (2002) Clin Lab Haematol 24: 225-32 [PubMed]
Serebruany (2006) J Thromb Haemost 4:1429-31 [PubMed]
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