Lab

Skin Test for Horse Serum Sensitivity

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Skin Test for Horse Serum Sensitivity, Horse Serum Sensitivity, Serum Sickness

  • Indication
  1. Skin Test for Horse Serum Sensitivity prior to use
  2. Many antitoxins and antivenins are from horse serum
  • Consider alternatives to testing
  1. Advantages to performing skin test prior to antivenin
    1. Possible medicolegal protection only
  2. Disadvantages to skin test prior to antitoxin
    1. Delays treatment (antitoxin administration)
    2. High false-positive rate: 33%
    3. High false-negative rate: 10 to 36%
    4. Has resulted in Anaphylaxis in some patients
  3. References
    1. Jurkovich (1998) J Trauma 28:1032-7 [PubMed]
  • Technique
  1. 0.1 ml of 1:10 dilution of antitoxin intradermal
  2. Inject at Forearm with 27 gauge
  • Observe for Allergic Reaction for 20 minutes
  1. Local reaction
    1. Hyperemic areola at injection site > 0.5 cm
  2. Systemic reaction
    1. Fever, chills
    2. Hypotension with decrease Blood Pressure >20 mmHg
    3. Skin rash
    4. Respiratory difficulty
    5. Nausea, Vomiting
    6. Generalized itching
  • Interpretation
  • If any signs of reaction
  1. Suggests Horse Serum Sensitivity
  2. Do not use antitoxin