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Bordetella Pertussis Test
Aka: Bordetella Pertussis Test, Whooping Cough Test, Pertussis Diagnostics, Pertussis Culture, Pertussis PCR, Bordatella PCR
- Technique: Obtaining Samples
- Dacron Polyester swab of posterior nasopharynx
- Insert into posterior nostril
- Leave swab in place for 10 seconds
- Do not swab anterior nare or throat (low efficacy)
- Nasopharyngeal aspirate
- Higher efficacy than swab
- Requires special equipment
- Labs: Tests Recommended by CDC (obtain both PCR and culture)
- Nasopharyngeal swab PCR
- Indicated as first-line test for Pertussis
- Test Sensitivity: 94%
- Test Specificity: 97%
- Result returned in 1-2 days
- Pertussis Culture was previously recommended by CDC for confirmation
- PCR is now considered accurate enough for diagnosis without confirmatory culture
- Pertussis Culture
- Previously indicated for confirmation of positive PCR (but PCR alone is now considered sufficient)
- Test Specificity: 100%
- Test Sensitivity
- Overall: 15%
- Early disease: 80%
- After Erythromycin for 4 days: approaches 0%
- Result returned in 7-12 days
- Labs: Tests Not recommended by CDC
- Pertussis Direct Fluorescent Antibody (DFA)
- Pertussis Serology (not standardized)
- References
- Gregory (2006) Am Fam Physician 74:420-7 [PubMed]
- Loeffelholz (1999) J Clin Microbiol 37:2872-6 [PubMed]
- Kline (2013) Am Fam Physician 88(8): 507-14 [PubMed]