Dermatology Book


Fungal Culture

Aka: Fungal Culture, Fungus Culture
  1. See Also
    1. Potassium Hydroxide (KOH Preparation)
    2. Onychomycosis
    3. Tinea Capitis
  2. Indication
    1. Suspected fungal infection (KOH Preparation negative)
      1. Onychomycosis (Tinea Unguium)
      2. Tinea Capitis
    2. Sensitivity to Antifungal Medications
  3. Approach: General
    1. Submit large amounts of debris
    2. Break up large pieces
    3. Collect in sterile urine cup or similar container
  4. Technique: Specimen collection
    1. Scaly Rash
      1. Collect scale as done in KOH Preparation
      2. Implant resulting scales on medium
    2. Non-Scaling Rash
      1. Wet cotton swab rubbed vigorously over lesion border
      2. Collect resulting debris into medium
    3. Nails
      1. See Onychomycosis
      2. Clip nails back to yellowish, crumbly portion
      3. Clip a sliver of crumbly portion and place on medium
      4. Gently scrape debris from under free edge of nail
    4. Scalp
      1. See Tinea Capitis
      2. Gently scrub area with sterile ToothBrush
        1. Tap ToothBrush bristles on medium
      3. Scrape a few scales off scalp for culture
      4. Gently pluck short stumps of hair and place on medium
  5. Preparations: Culture Medium
    1. Medium: Sabouraud's Peptone-Glucose Agar (most common)
    2. Additives: Dermatophyte test medium (DTM)
      1. Phenol indicator turns red when alkaline
      2. Poor sensitivity and Specificity
  6. Technique: Culture Identification
    1. Dermatophyte growth and identification takes 4-6 weeks
      1. Minimum culture time to call negative: 21 days
    2. Keep culture bottle at room Temperature
    3. Do not tightly cap bottles (loose enough to rattle)
      1. Dermatophytes are aerobes

You are currently viewing the original '\legacy' version of this website. Internet Explorer 8.0 and older will automatically be redirected to this legacy version.

If you are using a modern web browser, you may instead navigate to the newer desktop version of fpnotebook. Another, mobile version is also available which should function on both newer and older web browsers.

Please Contact Me as you run across problems with any of these versions on the website.

Navigation Tree