II. Indications

  1. Confirmation of an indeterminate or positive less-specific lab result (e.g. ELISA)

III. Mechanism

  1. Detects proteins and peptides separated by gel electrophoresis and identified with labeled antibodies

IV. Technique

  1. Sampled cells are lysed and protein extracted (e.g. from patient blood sample)
  2. Extracted proteins are applied to a gel surface
  3. Electrical current applied to gel matrix separates molecules based on charge and mass
  4. Separated molecules are transferred from gel onto paper or nylon membrane (e.g. nitrocellulose)
  5. Radiolabeled or Enzyme-linked Antibody probes (similar to ELISA) detect specific substances

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Ontology: Western Blotting (C0005863)

Definition (NCI_CDISC) A method for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes.
Definition (NCI) A laboratory procedure for the detection or identification of proteins or peptides that have been separated by gel electrophoresis and transferred onto nitrocellulose or other type of paper or nylon membrane. The proteins are then detected by reaction with enzymatically labeled or radiolabeled antibody probes.
Definition (MSH) Identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Definition (CSP) identification of proteins or peptides that have been electrophoretically separated by blot transferring from the electrophoresis gel to strips of nitrocellulose paper, followed by labeling with antibody probes.
Concepts Molecular Biology Research Technique (T063) , Laboratory Procedure (T059)
MSH D015153
English Blotting, Western, Blottings, Western, Immunoblotting, Western, Immunoblottings, Western, Western Blottings, Western Immunoblotting, Western Immunoblottings, Western Blot, Western Blotting, protein blotting, Western blotting, western blotting, blotting western, Western immunoblotting, WESTERN BLOT
Swedish Blotting, Western
Czech západní přenos, blotting western
Finnish Western blotting
French Technique de Western blot, Transfert de Western, Western, Buvardage de Western, Technique de Western
German Blotting, Western-, Immunblotting, Western-, Western-Blotting, Western-Immunblotting
Italian Western immunoblotting, Western blotting
Russian IMMUNOBLOTTING ZAPADNYI, ZAPADNYI IMMUNOBLOTTING, BLOTTING-VESTERN, VESTERN-BLOTTING, БЛОТТИНГ-ВЕСТЕРН, ВЕСТЕРН-БЛОТТИНГ, ЗАПАДНЫЙ ИММУНОБЛОТТИНГ, ИММУНОБЛОТТИНГ ЗАПАДНЫЙ
Japanese ウエスタン免疫ブロット法, ブロッティング-ウエスタン, 免疫ブロット法-ウエスタン, ウエスタンブロッティング
Polish Metoda western blot, Western blotting, Próba western blot, Blotting western, Test western blot, Blotting białek
Dutch Blotting, Western, Immunoblotting, Western, Western blotting
Portuguese Western Blot, Western Blotting, Western Immunoblotting
Spanish Western Blot, Western Blotting, Western Immunoblotting