Pulmonology Book

http://www.fpnotebook.com/

Bordetella Pertussis Test

Aka: Bordetella Pertussis Test, Whooping Cough Test, Pertussis Diagnostics, Pertussis Culture, Pertussis PCR
Advertisement
  1. Technique: Obtaining Samples
    1. Polyester swab of nasopharynx
      1. Insert into base of nostril
      2. Leave swab in place for 10 seconds
    2. Nasopharyngeal aspirate
      1. Higher efficacy than swab
      2. Requires special equipment
  2. Tests: Recommended by CDC
    1. Nasopharyngeal swab PCR (preferred)
      1. Indicated as first-line test for Pertussis
      2. Test Sensitivity: 94%
      3. Test Specificity: 97%
      4. Result returned in 1-2 days
      5. Pertussis Culture recommended by CDC for confirmation
    2. Pertussis Culture (losing favor)
      1. Indicated for confirmation of positive PCR
      2. Test Specificity: 100%
      3. Test Sensitivity
        1. Overall: 15%
        2. Early disease: 80%
        3. After Erythromycin for 4 days: approaches 0%
      4. Result returned in 7-12 days
  3. Tests: Not recommended by CDC
    1. Pertussis Direct Fluorescent Antibody (DFA)
    2. Pertussis Serology (not standardized)
  4. References
    1. Gregory (2006) Am Fam Physician 74:420-7
    2. Loeffelholz (1999) J Clin Microbiol 37:2872-6

Navigation Tree